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Anti-Growth/differentiation factor 5 (GDF5) Antibody, Biotinylated
Catalog #: 130-00006B-50
Chicken anti-Growth/differentiation factor 5 (GDF5) Biotinylated Antibody, 50 ug
Product Description
Specifications
| Size | 50 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Host Species | Chicken |
| Accession Number | NP_000548 |
| Immunogen | Immunogen was synthetic peptide derived from Human GDF5. This antibody was produced from a chicken immunized with the immunogen. The IgY fraction was purified from collected egg followed by in-house affinity chromatography. |
| Isotype | IgY |
| Recommended Applications | ELISA (recommended work dilution= 1: 1, 000-5, 000), Western Blotting (recommended work dilution= 1: 1, 000-2, 000) |
| Reconstitution | A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial. |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Description
Description
Growth/differentiation factor 5 (GDF5) is a protein that in humans is encoded by the GDF5 gene. The protein encoded by this gene is closely related to the bone morphogenetic protein (BMP) family and is a member of the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Mutations in this gene are associated with acromesomelic dysplasia, HunterThompson type; brachydactyly, type C; and chondrodysplasia, Grebe type. These associations confirm that the gene product plays a role in skeletal development
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
The chicken anti-Human GDF5 antibody specifically detected target derived from human recombinant protein as well as selected cell lysates. Cross reactivity with mouse and rat GDF5 has not yet been tested.
References
- H¶tten G, Neidhardt H, Jacobowsky B, Pohl J (1994). "Cloning and expression of recombinant human growth/differentiation factor 5.". Biochem. Biophys. Res. Commun. 204 (2): 64652
- Nishitoh H, Ichijo H, Kimura M, et al. (1996). "Identification of type I and type II serine/threonine kinase receptors for growth/differentiation factor-5.". J. Biol. Chem. 271 (35): 2134552.
Expiration:
12 months from the date of shipment when stored properly.