Anti-GSTP1 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P09211
Gene Id	2950
Gene Symbols	GSTP1
Protein Name / Synonyms	GSTP1 (Center)
Clonality	Polyclonal
Preparation	This GSTP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97-126 amino acids from the Central region of human GSTP1.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Research Area	Cancer, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. Based on their biochemical, immunologic, and structural properties, the soluble GSTs are categorized into 4 main classes: alpha, mu, pi, and theta. This GST family member is a polymorphic gene encoding active, functionally different GSTP1 variant proteins that are thought to function in xenobiotic metabolism and play a role in susceptibility to cancer, and other diseases.

Specificity

Other Names

GSTP1; FAEES3; GST3; Glutathione S-transferase P; GST class-pi; GSTP1-1

NCBI Accession #

NP_000843.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

23356

Recommended Dilutions

WB: 1:1000; IF: 1:10-50; IHC: 1:50-100; FC: 1:10-50

	GSTP1 Antibody (Center) (Cat# 102-18186) western blot analysis in K562,PC-3,Y79 cell line and mouse lung tissue lysates (35ug/lane).This demonstrates the GSTP1 antibody detected the GSTP1 protein (arrow).
	Fluorescent confocal image of A549 cell stained with GSTP1 Antibody (Center)(Cat# 102-18186).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with GSTP1 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). GSTP1 immunoreactivity is localized to Cytoplasm and Mitochondria significantly.
	Fluorescent confocal image of A549 cell stained with GSTP1 Antibody (Center)(Cat# 102-18186).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with GSTP1 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). GSTP1 immunoreactivity is localized to Cytoplasm significantly.
	GSTP1 Antibody (Center) (RB20807) IHC analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GSTP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
	GSTP1 Antibody (Center) (Cat# 102-18186) flow cytometric analysis of Ramos cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.