Anti-H-Ras Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Rat
Host Species	Rabbit
Gene Id	3265
Protein Name / Synonyms	H-Ras
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Research Area	Ras/Rap Signaling
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

C-BAS/HAS antibody, C-H-RAS antibody, C-HA-RAS1 antibody, CTLO antibody, H-RASIDX antibody, HAMSV antibody, HRAS1 antibody, K-RAS antibody, N-RAS antibody, RASH1 antibody, HRAS antibody, transforming protein p21 antibody, Ha-Ras1 proto-oncoprotein antibody, H-Ras-1 antibody, GTPase HRas antibody, c-has/bas p21 protein antibody, transformation gene: oncogene HAMSV antibody, p19 H-RasIDX protein antibody, GTP- and GDP-binding peptide B antibody, p21ras antibody, Ras family small GTP binding protein H-Ras antibody, ha-Ras antibody, c-ras-Ki-2 activated oncogene antibody, v-Ha-ras Harvey rat sarcoma viral oncogene homolog antibody

Specificity

Species Reactivity

Human, Rat

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

21 kDa

Immunogen

Synthetic peptide corresponding to a region within amino acids 111 and 189 of H-Ras (Uniprot ID# P01112)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

H-Ras antibody detects HRAS protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
12 % SDS-PAGE
H-Ras antibody (107-11169) dilution: 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using H-Ras (107-11169) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft , using H-Ras (107-11169) antibody at 1:500 dilution.

Sample (30 ug of whole cell lysate)
A: Hela
B: Hep G2
C: Molt-4
12% SDS PAGE
107-11169 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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