Anti-H2AFX (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Accession Number	P16104
Gene Id	3014
Gene Symbols	H2AFX
Protein Name / Synonyms	H2AFX (N-term)
Clonality	Monoclonal
Preparation	This H2AFX antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human H2AFX.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C-terminal phosphorylation.

Specificity

Predicted Reactivity

Mouse, Zebrafish

Other Names

H2AFX;H2AX; Histone H2A.x

NCBI Accession #

NP_002096.1

Other Accession #

P27661; Q7ZUY3

Format

Type

Mouse Monoclonal Antibody (Mab)

Calculated Molecular Weight (Da)

15145

Recommended Dilutions

WB: 1:1000

H2AFX Antibody (N-term) (Cat# 102-10421) western blot analysis in CEM,HepG2,Raji cell line lysates (35ug/lane).This demonstrates the H2AFX antibody detected the H2AFX protein (arrow).

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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