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Anti-H3K79me2 Antibody
Catalog #: 144-61341
DiMethyl-Histone H3-K79 pAb
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q16695 |
| Gene Id | 8290 |
| Gene Symbols | H3K79ME2 |
| Protein Name / Synonyms | HIST3H3, H3.4, H3/g, H3FT, H3t, histone H3.1t |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP, ChIP, ChIPseq |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Dilutions
WB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:50 - 1:200
CHIPseq 1:50 - 1:200
IHC 1:50 - 1:100
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:50 - 1:200
CHIPseq 1:50 - 1:200
Format
Calculated MW
15kDa
Observed MW
17kDa
Immunogen
A synthetic peptide of human DiMethyl-Histone H3-K79
Modification
DiMethyl K79
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K79 antibody (144-61341).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K79 antibody (144-61341).
Immunohistochemistry of paraffin-embedded rat kidney using DiMethyl-Histone H3-K79 antibody (144-61341) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using DiMethyl-Histone H3-K79 antibody (144-61341) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded Mouse heart using DiMethyl-Histone H3-K79 antibody (144-61341) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K79 antibody (144-61341). Blue: DAPI for nuclear staining.
Chromatin Immunoprecipitation analysis of ?-actin gene from 293 cell line, using DiMethyl-Histone H3-K79 antibody (144-61341) and rabbit IgG. P1, P2 and P3 were probes located on ?-actin gene as the schematic diagram illustrated. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Expiration:
12 months from the date of shipment when stored properly.