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Anti-H4K16ac Antibody
Catalog #: 144-61412
Acetyl-Histone H4-K16 pAb
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P62805 |
| Gene Id | 8370 |
| Gene Symbols | H4K16AC |
| Protein Name / Synonyms | HIST2H4A, FO108, H4, H4/n, H4F2, H4FN, HIST2H4, histone H4 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, ChIP |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy.
Dilutions
WB 1:500 - 1:2000
ChIP 1:50 - 1:200
ChIP 1:50 - 1:200
Format
Calculated MW
11kDa
Observed MW
13kDa
Immunogen
A synthetic acetylated peptide around K16 of human Histone H4 (NP_001029249.1).
Modification
Acetyl K16
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K16 antibody (144-61412) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Expiration:
12 months from the date of shipment when stored properly.