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Anti-HA-Tag Antibody, Biotinylated

Catalog #: RB-IP-10-101B-50

Mouse anti-HA-Tag Biotinylated Antibody, 50 ug

Product Description

Specifications

Size50 µg
Estimated Lead Time1-2 business days
Specificity
HA-Tag antibody detects fusion recombinant proteins derived from a variety of sources at various concentrations. No Cross reactivity with cell lysates was not found in ELISA and Western Blot.
Host SpeciesMouse
Protein Name / Synonyms
HA-Tag
ClonalityMonoclonal
Immunogen
Conjugated-Peptide with sequence YPYDVPDYA was used as immunogen. The antibody was purified from cell culture supernatantby affinity chromatography.
IsotypeIgG1
Recommended ApplicationsELISA (recommended work dilution= 1:1000-5000), Western Blotting( recommended work dilution= 1-2 µg/ml)
Reconstitution
A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial.
Shipping TypeAmbient temperature
Storage-20°C

Description

Description

Plasmid vectors are commonly used for the expression of hybrid fusion proteins, encoding a protein of interest fused to an epitope tag. Antibodies specific for such fusion protein tags are useful for monitoring expression of the protein of interest, both in cells and in Western blot analysis. Such epitope tags in common use include Myc, GST, His, HA and GFP. HA Tag is very common using epitope tags. The tags are encoded by a variety of expression vectors, and effective vectors suitable for the production of recombinant tagged fusion proteins. Each expression vector encodes a unique, nine amino acids HATag epitope sequence. Anti-HA-Tag antibodies are suitable for purification, detection and immunoprecipitation of recombinant fusion proteins containing the respective HA-Tag.

Storage / Stability

The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.

Specificity

HA- Tag antibody detects fusion recombinant proteins derived from a variety of sources at various concentrations. No Cross reactivity with cell lysates was not found in ELISA and Western Blot.

References

  • Yang RZ, et al..(2006) Identification of omentin as a novel depot-specific adipokine in human adipose tissue: possible role in modulating insulin action. Am J Physiol Endocrinol Metab. 290(6):E1253-61.
  • Sch¤ffler A, et al.. (2005) Genomic structure of human omentin, a new adipocytokine expressed in omental adipose tissue. Biochim Biophys Acta. 1732(1-3):96-102.
Expiration:
12 months from the date of shipment when stored properly.