Anti-HACE1 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q8IYU2
Gene Id	57531
Gene Symbols	HACE1
Protein Name / Synonyms	HACE1, SPPRS, E3 ubiquitin-protein ligase HACE1
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Background

This gene encodes a HECT domain and ankyrin repeat-containing ubiquitin ligase. The encoded protein is involved in specific tagging of target proteins, leading to their subcellular localization or proteasomal degradation. The protein is a potential tumor suppressor and is involved in the pathophysiology of several tumors, including Wilm's tumor.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200

Format

Calculated MW

35kDa/63kDa/77kDa/102kDa

Observed MW

102kDa

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HACE1 (NP_065822.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using HACE1 antibody (144-61480) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - HACE1 Polyclonal Antibody (144-61480)

Expiration:

12 months from the date of shipment when stored properly.

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