Rabbit Anti-Human HAND2 (Center) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P61296 |
| Gene Id | 9464 |
| Gene Symbols | HAND2 |
| Protein Name / Synonyms | HAND2 (Center) |
| Clonality | Polyclonal |
| Preparation | This HAND2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 81-110 amino acids from the Central region of human HAND2. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Research Area | Angiogenesis, Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, this transcription factor plays an important role in limb and branchial arch development.
Specificity
Predicted Reactivity
Mouse, Rat, Chicken, Xenopus
Other Names
HAND2; BHLHA26; DHAND; Heart- and neural crest derivatives-expressed protein 2; Class A basic helix-loop-helix protein 26; Deciduum, heart, autonomic nervous system and neural crest derivatives-expressed protein 2
NCBI Accession #
NP_068808.1
Other Accession #
P57101; P61295; Q61039; Q90690; NP_068808.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
23666
Recommended Dilutions
IF: 1:10-50; WB: 1:1000
 | Fluorescent confocal image of Hela cell stained with HAND2 Antibody (Center)(Cat# 102-13234).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HAND2 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).HAND2 immunoreactivity is localized to Nucleus significantly. |
 | HAND2 Antibody (Center) (Cat# 102-13234) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the HAND2 antibody detected the HAND2 protein (arrow). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
