Anti-HBA2 (Center) Antibody

Product Description

Specifications

Size	200 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P69905
Gene Id	3040
Gene Symbols	HBA1
Protein Name / Synonyms	HBA2 (Center)
Clonality	Polyclonal
Preparation	This HBA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 100-128 amino acids from the Central region of human HBA2.
Concentration (lot specific)	0.460 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Cardiovascular Disease, Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

HBA2 located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.

Specificity

Predicted Reactivity

Monkey

Other Names

HBA1; Hemoglobin subunit alpha; Alpha-globin; Hemoglobin alpha chain

NCBI Accession #

NP_000508.1;NP_000549.1

Other Accession #

P21767

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

15258

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of HBA2 Antibody (Center) (Cat# 102-18267) in mouse liver tissue lysates (35ug/lane). HBA2 (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HBA2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	HBA2 Antibody (Center) (Cat# 102-18267) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products