Anti-HDAC1 (Center S423) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q13547
Gene Id	3065
Gene Symbols	HDAC1
Protein Name / Synonyms	HDAC1 (Center S423)
Clonality	Polyclonal
Preparation	This HDAC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 402-430 amino acids from the Central region of human HDAC1.
Concentration (lot specific)	0.450 mg/ml
Recommended Applications	Western Blotting, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.

Specificity

Other Names

HDAC1; RPD3L1; Histone deacetylase 1

NCBI Accession #

NP_004955.2

Other Accession #

NP_004955.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

55103

Recommended Dilutions

WB: 1:1000; IF: 1:10-50

HDAC1 Antibody (S423) (Cat# 102-13174) western blot analysis in Hela cell line and mouse testis,rat testis tissue lysates (35ug/lane).This demonstrates the HDAC1 antibody detected the HDAC1 protein (arrow).

Fluorescent confocal image of Hela cell stained with HDAC1 Antibody (Center S423)(Cat# 102-13174).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC1 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HDAC1 immunoreactivity is localized to Nucleus significantly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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