Anti-HDAC4 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P56524
Gene Id	9759
Gene Symbols	HDAC4
Protein Name / Synonyms	HDAC4, AHO3, BDMR, HA6116, HD4, HDAC-4, HDAC-A, HDACA, histone deacetylase 4
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF, IP
Research Area	Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:100

Format

Calculated MW

106kDa/119kDa

Observed MW

117kDa

Immunogen

A synthetic peptide of human HDAC4

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using HDAC4 antibody (144-00239) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - HDAC4 Polyclonal Antibody (144-00239)

Immunofluorescence analysis of A549 cells using HDAC4 antibody (144-00239). Blue: DAPI for nuclear staining.
Immunofluorescence - HDAC4 Polyclonal Antibody (144-00239)

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug HDAC4 antibody (144-00239). Western blot was performed from the immunoprecipitate using HDAC4 antibody (144-00239) at a dilition of 1:1000.
Immunoprecipitation - HDAC4 Polyclonal Antibody (144-00239)

Expiration:

12 months from the date of shipment when stored properly.

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