Anti-HDAC4 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P56524
Gene Id	9759
Gene Symbols	HDAC4
Protein Name / Synonyms	HDAC4 (C-term)
Clonality	Polyclonal
Preparation	This HDAC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1049-1077 amino acids from the C-terminal region of human HDAC4.
Concentration (lot specific)	0.350 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It possesses histone deacetylase activity and represses transcription when tethered to a promoter. This protein does not bind DNA directly, but through transcription factors MEF2C and MEF2D. It seems to interact in a multiprotein complex with RbAp48 and HDAC3.

Specificity

Other Names

HDAC4; KIAA0288; Histone deacetylase 4

NCBI Accession #

NP_006028.2

Other Accession #

NP_006028.2

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

119040

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50

	HDAC4 Antibody (C-term) (Cat# 102-12117) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the HDAC4 antibody detected the HDAC4 protein (arrow).
	HDAC4 Antibody (C-term) (Cat# 102-12117)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HDAC4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	Fluorescent confocal image of Hela cell stained with HDAC4 Antibody (C-term)(Cat# 102-12117).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC4 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HDAC4 immunoreactivity is localized to Nucleus significantly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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