Anti-HDAC6 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9UBN7
Gene Id	10013
Gene Symbols	HDAC6
Protein Name / Synonyms	HDAC6, CPBHM, HD6, JM21, PPP1R90, histone deacetylase 6
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF, IP
Research Area	Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:20 - 1:50

Format

Calculated MW

114kDa/131kDa

Observed MW

170kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 836-1104 of human HDAC6 (NP_006035.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using HDAC6 antibody (144-11259) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - HDAC6 Polyclonal Antibody (144-11259)

Immunofluorescence analysis of HeLa cells using HDAC6 antibody (144-11259) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence - HDAC6 Polyclonal Antibody (144-11259)

Immunoprecipitation analysis of 100ug extracts of HepG2 cells using 3ug HDAC6 antibody (144-11259). Western blot was performed from the immunoprecipitate using HDAC6 antibody (144-11259) at a dilition of 1:1000.
Immunoprecipitation - HDAC6 Polyclonal Antibody (144-11259)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products