Anti-HDAC7 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q8WUI4
Gene Id	51564
Gene Symbols	HDAC7
Protein Name / Synonyms	HDAC7, HD7, HD7A, HDAC7A, histone deacetylase 7
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IF
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to mouse HDAC7 gene whose protein promotes repression mediated via the transcriptional corepressor SMRT. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IF 1:50 - 1:100

Format

Calculated MW

46-66kDa/99-108kDa

Observed MW

120kDa

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 400-500 of human HDAC7 (NP_056216.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using HDAC7 antibody (144-07285) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - HDAC7 antibody (144-07285)

Immunofluorescence analysis of MCF-7 cells using HDAC7 antibody (144-07285). Blue: DAPI for nuclear staining.
Immunofluorescence - HDAC7 antibody (144-07285)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products