Anti-HDAC8 Antibody

Product Description

Specifications

Size	20 µL, 100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Accession Number	Q9BY41
Gene Id	55869
Gene Symbols	HDAC8
Protein Name / Synonyms	HDAC8, CDA07, CDLS5, HD8, HDACL1, MRXS6, RPD3, WTS, histone deacetylase 8
Clonality	Polyclonal
Isotype	IgG
Purity	Affinity purification
Buffers	PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Recommended Applications	Western Blotting, IHC, IF
Shipping Type	Blue ice
Storage	-20°C

Description

Background

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class I of the histone deacetylase family. It catalyzes the deacetylation of lysine residues in the histone N-terminal tails and represses transcription in large multiprotein complexes with transcriptional co-repressors. Multiple transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200

Format

Calculated MW

15kDa/16kDa/17kDa/28kDa/29kDa/31kDa/41kDa

Observed MW

42kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-377 of human HDAC8 (NP_060956.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using HDAC8 Antibody (144-05829) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - [KO Validated] HDAC8 Polyclonal Antibody (144-05829)

Western blot analysis of extracts from normal (control) and HDAC8 knockout (KO) HeLa cells, using HDAC8 antibody (144-05829) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Western blot - [KO Validated] HDAC8 Polyclonal Antibody (144-05829)

Immunohistochemistry of paraffin-embedded rat testis using HDAC8 antibody (144-05829) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] HDAC8 Polyclonal Antibody (144-05829)

Immunohistochemistry of paraffin-embedded rat spinal cord using HDAC8 antibody (144-05829) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] HDAC8 Polyclonal Antibody (144-05829)

Immunohistochemistry of paraffin-embedded human breast cancer using HDAC8 antibody (144-05829) at dilution of 1:100 (40x lens).
Immunohistochemistry - [KO Validated] HDAC8 Polyclonal Antibody (144-05829)

Expiration:

12 months from the date of shipment when stored properly.

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