Rabbit Anti-Human HEYL (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q9NQ87 |
| Gene Id | 26508 |
| Gene Symbols | HEYL |
| Protein Name / Synonyms | HEYL (N-term) |
| Clonality | Polyclonal |
| Preparation | This HEYL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-54 amino acids from the N-terminal region of human HEYL. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
This gene encodes a member of the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcription factors. The sequence of the encoded protein contains a conserved bHLH and orange domain, but its YRPW motif has diverged from other HESR family members. It is thought to be an effector of Notch signaling and a regulator of cell fate decisions. Alternatively spliced transcript variants have been found, but their biological validity has not been determined. [provided by RefSeq].
Specificity
Predicted Reactivity
Mouse, Bovine
Other Names
HEYL; BHLHB33; HRT3; Hairy/enhancer-of-split related with YRPW motif-like protein; Class B basic helix-loop-helix protein 33; Hairy-related transcription factor 3
NCBI Accession #
NP_055386.1
Other Accession #
Q9DBX7; Q2NL18; NP_055386.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
35087
Recommended Dilutions
WB: 1:1000; IF: 1:10-50
 | HEYL Antibody (N-term) (Cat# 102-13226) western blot analysis in MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HEYL antibody detected the HEYL protein (arrow). |
 | Fluorescent confocal image of Hela cell stained with HEYL Antibody (N-term)(Cat# 102-13226).HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HEYL primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HEYL immunoreactivity is localized to Mitochondria significantly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
