Anti-HGF (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P14210
Gene Id	3082
Gene Symbols	HGF
Protein Name / Synonyms	HGF (C-term)
Clonality	Polyclonal
Preparation	This HGF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 521-554 amino acids from the C-terminal region of human HGF.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Inflammation, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. Transcript Variant: This variant (1) encodes the longest isoform (1). To date, experimental evidence for cleavage of the proprotein into two mature chains has been shown only for isoform 1.

Specificity

Other Names

HGF; HPTA; Hepatocyte growth factor; Hepatopoietin-A; Scatter factor; Hepatocyte growth factor alpha chain; Hepatocyte growth factor beta chain

NCBI Accession #

NP_000592.3;NP_001010931.1;NP_001010932.1;NP_001010933.1;NP_001010934.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

83134

Recommended Dilutions

WB: 1:1000; FC: 1:10-50; IHC: 1:50-100

	The anti-HGF Pab (Cat# 102-13335) is used in Western blot to detect HGF in Ramos tissue lysate. HGF (arrow) was detected using the purified Pab.
	HGF Antibody (C-term) (Cat# 102-13335) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
	Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HGF antibody (C-term) (Cat# 102-13335), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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