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Anti-HHIP (internal) Antibody
Catalog #: 107-11034
HHIP antibody [N3C2], Internal, 0.86 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Chicken, Human, Mouse |
| Host Species | Rabbit |
| Gene Id | 64399 |
| Protein Name / Synonyms | HHIP, Internal |
| Concentration (lot specific) | 0.86 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
FLJ20992 antibody, FLJ90230 antibody, HIP antibody, HHIP antibody, hedgehog-interacting protein antibody, hedgehog interacting protein antibody
Specificity
Species Reactivity
Human, Chicken, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
79 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 263 and 554 of HHIP (Uniprot ID# Q96QV1)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
HHIP antibody [N3C2], Internal antibody detects HHIP protein by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde for 15 min.
Green: HHIP protein stained by HHIP antibody [N3C2], Internal antibody (107-11034) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 ?m.
Sample: A431 cells were fixed in 4% paraformaldehyde for 15 min.
Green: HHIP protein stained by HHIP antibody [N3C2], Internal antibody (107-11034) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 ?m.
HHIP antibody [N3C2], Internal detects HHIP protein at cytosol on hepatoma by immunohistochemical analysis.
Sample: Paraffin-embedded hepatoma.
HHIP antibody [N3C2], Internal (107-11034) dilution: 1:500.
Sample: Paraffin-embedded hepatoma.
HHIP antibody [N3C2], Internal (107-11034) dilution: 1:500.
Sample (50 ug of whole cell lysate)
A: Mouse brain
7.5% SDS PAGE
107-11034 diluted at 1:1000
A: Mouse brain
7.5% SDS PAGE
107-11034 diluted at 1:1000
Sample (30 ug of whole cell lysate)
A: A431
7.5% SDS PAGE
107-11034 diluted at 1:1000
A: A431
7.5% SDS PAGE
107-11034 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.