Anti-HIF1Alpha (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q16665
Gene Id	3091
Gene Symbols	HIF1A
Protein Name / Synonyms	HIF1Alpha (C-term)
Clonality	Polyclonal
Preparation	This HIF1Alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 728-757 amino acids from the C-terminal region of human HIF1Alpha.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IF, IHC-P
Research Area	Angiogenesis, Cardiovascular Disease, Inflammation, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT).

Specificity

Other Names

HIF1A; BHLHE78; MOP1; PASD8; Hypoxia-inducible factor 1-alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; Member of PAS protein 1; PAS domain-containing protein 8

NCBI Accession #

NP_001230013.1;NP_001521.1;NP_851397.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

92670

Recommended Dilutions

WB: 1:1000; IF: 1:10-50; IHC: 1:10-50

	Western blot analysis of HIF1Alpha Antibody (C-term) (Cat# 102-17607) in MDA-MB231 cell line lysates (35ug/lane). HIF1Alpha (arrow) was detected using the purified Pab.
	Western blot analysis of HIF1Alpha Antibody (C-term) (Cat# 102-17607) in mouse cerebellum tissue lysates (35ug/lane). HIF1Alpha (arrow) was detected using the purified Pab.
	Confocal immunofluorescent analysis of HIF1Alpha Antibody (C-term)(Cat# 102-17607) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
	Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HIF1Alpha antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohis

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

Featured Products