Anti-HIF1Alpha (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	Q16665
Gene Id	3091
Gene Symbols	HIF1A
Protein Name / Synonyms	HIF1Alpha (Center)
Clonality	Polyclonal
Preparation	This HIF1Alpha antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 519-547 amino acids from the Central region of human HIF1Alpha.
Concentration (lot specific)	2.000 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Research Area	Angiogenesis, Cardiovascular Disease, Inflammation, Autophagy
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia. HIF1 is a heterodimer composed of an alpha subunit and a beta subunit. The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT). This gene encodes the alpha subunit of HIF-1. Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas.

Specificity

Predicted Reactivity

Rat, Bovine

Other Names

HIF1A; BHLHE78; MOP1; PASD8; Hypoxia-inducible factor 1-alpha; ARNT-interacting protein; Basic-helix-loop-helix-PAS protein MOP1; Class E basic helix-loop-helix protein 78; Member of PAS protein 1; PAS domain-containing protein 8

NCBI Accession #

NP_001230013.1;NP_001521.1;NP_851397.1

Other Accession #

O35800; Q61221; Q9XTA5

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

92670

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; IF: 1:10-50; FC: 1:10-50

	Western blot analysis of HIF1Alpha Antibody (Center) (Cat# 102-17608) in mouse NIH-3T3 cell line lysates (35ug/lane). HIF1Alpha (arrow) was detected using the purified Pab.
	HIF1Alpha Antibody (Center)(Cat# 102-17608) IHC analysis in formalin fixed and paraffin embedded lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HIF1Alpha Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of HIF1Alpha Antibody (Center)(Cat# 102-17608) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
	HIF1Alpha Antibody (Center) (Cat# 102-17608) flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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