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Anti-HIF1AN (C-term) Antibody
Catalog #: 102-20296
Mouse Anti-HIF1AN (C-term) Antibody, 200 µl
Product Description
Specifications
| Size | 200 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Mouse |
| Accession Number | Q9NWT6 |
| Gene Id | 55662 |
| Gene Symbols | HIF1AN |
| Protein Name / Synonyms | Hypoxia-inducible factor 1-alpha inhibitor, 1.14.11.30, 1.14.11.n4, Factor inhibiting HIF-1, FIH-1, Hypoxia-inducible factor asparagine hydroxylase, HIF1AN, FIH1 |
| Clonality | Monoclonal |
| Clone | 931CT3.1.1 |
| Isotype | Kappa, IgG1 |
| Recommended Applications | IF, Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
Hydroxylates HIF-1 alpha at 'Asp-803' in the C-terminal transactivation domain (CAD). Functions as an oxygen sensor and, under normoxic conditions, the hydroxylation prevents interaction of HIF-1 with transcriptional coactivators including Cbp/p300- interacting transactivator. Involved in transcriptional repression through interaction with HIF1A, VHL and histone deacetylases. Hydroxylates specific Asn residues within ankyrin repeat domains (ARD) of NFKB1, NFKBIA, NOTCH1, ASB4, PPP1R12A and several other ARD-containing proteins. Also hydroxylates Asp and His residues within ARDs of ANK1 and TNKS2, respectively. Negatively regulates NOTCH1 activity, accelerating myogenic differentiation. Positively regulates ASB4 activity, promoting vascular differentiation.
Other Information
Antigen Type
Recombinant Protein
Format
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.
Calculated Molecular Weight (Da)
40285
Antigen Source
Human
Images
IF (1:25)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling HIF1AN with 102-20296 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG (NH174309) secondary antibody at 1/200 dilution (green). Immunofluorescence image showing mitochondrion staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin (OI17558410) at 1/100 dilution (red). The nuclear counter stain is DAPI (blue).
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.