Anti-HIP2 (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P61086
Gene Id	3093
Gene Symbols	UBE2K
Protein Name / Synonyms	HIP2 (Center)
Clonality	Polyclonal
Preparation	This HIP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 109-139 amino acids from the Central region of human HIP2.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF
Research Area	Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

HIP2 belongs to the ubiquitin-conjugating enzyme family. It binds selectively to a large region at the N terminus of huntingtin. This interaction is not influenced by the length of the huntingtin polyglutamine tract. This protein has been implicated in the degradation of huntingtin and suppression of apoptosis.

Specificity

Predicted Reactivity

Bovine

Other Names

UBE2K; HIP2; LIG; Ubiquitin-conjugating enzyme E2 K; Huntingtin-interacting protein 2; Ubiquitin carrier protein; Ubiquitin-conjugating enzyme E2-25 kDa; Ubiquitin-protein ligase

NCBI Accession #

NP_001104582.1;NP_001104583.1;NP_005330.1

Other Accession #

P61087; P61085; NP_005330

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

22407

Recommended Dilutions

IF: 1:10-50; WB: 1:1000

Fluorescent image of Hela cell stained with HIP2 Antibody (Center)(Cat# 102-14970/SH030911H).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HIP2 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°). HIP2 immunoreactivity is localized to Cytoplasm significantly.

HIP2 Antibody (D124) (Cat# 102-14970) western blot analysis in 293,mouse C2C12 cell line lysates (35ug/lane).This demonstrates the HIP2 antibody detected the HIP2 protein (arrow).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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