Anti-HIST1H2AB (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P04908
Gene Id	8335
Gene Symbols	HIST1H2AB
Protein Name / Synonyms	HIST1H2AB (N-term)
Clonality	Polyclonal
Preparation	This HIST1H2AB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-40 amino acids from the N-terminal region of human HIST1H2AB.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.

Specificity

Predicted Reactivity

Mouse, Rat, Zebrafish, Bovine, Chicken, Monkey, Xenopus

Other Names

HIST1H2AB; H2AFM; Histone H2A type 1-B/E; Histone H2A.2; Histone H2A/a; Histone H2A/m

NCBI Accession #

NP_003504.2;NP_066390.1

Other Accession #

Q6GM86; Q7ZUY3; A9UMV8; Q8R1M2; Q4R3X5; Q9BTM1; P70082; Q3ZBX9; P02263; Q4FZT6; Q8BFU2; Q7L7L0; P35062; P06898; Q64523; Q16777; A1A4R1; Q64522; Q8IUE6; P0CC09; Q6GSS7; Q6FI13; P06897; P02262; P22752; P0C0S8; P0C0S9; Q8CGP7; Q99878; Q8CGP6; Q96KK5; Q64598;

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

14135

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; FC: 1:10-50

	HIST1H2AB Antibody (N-term) (Cat# 102-11853) western blot analysis in K562,CEM,MDA-MB453,NCI-H460,MDA-MB231 cell line lysates (35ug/lane).This demonstrates the HIST1H2AB antibody detected the HIST1H2AB protein (arrow).
	HIST1H2AB Antibody (N-term) (Cat# 102-11853)immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HIST1H2AB Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	HIST1H2AB Antibody (N-term) (Cat# 102-11853) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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