Anti-HIST1H2AH (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	Q96KK5
Gene Id	85235
Gene Symbols	HIST1H2AH
Protein Name / Synonyms	HIST1H2AH (N-term)
Clonality	Polyclonal
Preparation	This HIST1H2AH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human HIST1H2AH.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine, Chicken

Other Names

HIST1H2AH; HIST1H2AI; Histone H2A type 1-H; Histone H2A/s

NCBI Accession #

NP_542163.1

Other Accession #

P02263; Q4FZT6; Q8BFU2; Q7L7L0; P35062; Q64523; Q16777; A1A4R1; Q64522; Q8IUE6; P0CC09; Q6GSS7; Q6FI13; P02262; P22752; P0C0S8; P0C0S9; Q8CGP7; Q99878; Q8CGP6; Q64598; Q8CGP5; P0C170; P20671; P0C169; Q93077; P04908; NP_542163

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

13906

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	Western blot analysis of HIST1H2AH Antibody (N-term) (Cat# 102-18044) in A2058, A375, K562, Hela, MCF-7 cell line lysates (35ug/lane). HIST1H2AH (arrow) was detected using the purified Pab.
	Formalin-fixed and paraffin-embedded human brain tissue reacted with HIST1H2AH Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	HIST1H2AH Antibody (N-term) (Cat# 102-18044) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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