Anti-HIST1H2AL (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P0C0S8
Gene Id	8969
Gene Symbols	HIST1H2AG
Protein Name / Synonyms	HIST1H2AL (C-term)
Clonality	Polyclonal
Preparation	This HIST1H2AL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 103-130 amino acids from the C-terminal region of human HIST1H2AL.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3.

Specificity

Predicted Reactivity

Mouse, Rat, Bovine

Other Names

HIST1H2AG; H2AFP; Histone H2A type 1; Histone H2A/p

NCBI Accession #

NP_003500.1;NP_003501.1;NP_003502.1;NP_003505.1;NP_066408.1

Other Accession #

Q4FZT6; Q8BFU2; Q7L7L0; P0CC09; Q6GSS7; Q6FI13; P02262; P22752; P0C0S9; P0C170; P20671; P0C169; Q93077; P04908

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

14091

Recommended Dilutions

WB: 1:1000; IHC: 1:50-100; FC: 1:10-50

	HIST1H2AL Antibody (C-term) (Cat# 102-15788) western blot analysis in HL-60,HepG2,K562,CEM,MCF-7 cell line lysates (35ug/lane).This demonstrates the HIST1H2AL antibody detected the HIST1H2AL protein (arrow).
	HIST1H2AL Antibody (C-term) (Cat# 102-15788) IHC analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HIST1H2AL Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
	HIST1H2AL Antibody (C-term) (Cat# 102-15788) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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