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Anti-HIST1H2BJ (Center) Antibody

Catalog #: 102-10774

Rabbit Anti-Human HIST1H2BJ (Center) Antibody, 400 ul

Product Description

Specifications

Size400 µL
Estimated Lead Time1-2 weeks
SpeciesHuman
Host SpeciesRabbit
Accession Number
P06899
Gene Id
8970
Gene Symbols
HIST1H2BJ
Protein Name / Synonyms
HIST1H2BJ (Center)
ClonalityPolyclonal
PreparationThis HIST1H2BJ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-86 amino acids from the Central region of human HIST1H2BJ.
Concentration (lot specific)
0.5 mg/ml
Recommended ApplicationsIHC-P, FC, Western Blotting
Research AreaCancer
Shipping TypeBlue ice
Storage-20°C

Description

Introduction

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq].

Specificity

Predicted Reactivity
Mouse, Rat, Zebrafish, Bovine, Chicken, Monkey, Xenopus
Other Names
HIST1H2BJ; H2BFR; Histone H2B type 1-J; Histone H2B.1; Histone H2B.r
NCBI Accession #
NP_066402.2
Other Accession #
Q9PSW9; P0C1H5; P0C1H4; Q6PC60; Q5QNW6; Q16778; Q6DRA6; Q6DN03; Q64525; Q5BJA5; P0C1H3; P62808; P23527; Q99877; Q32L48; P10854; Q99879; Q2PFX4; O60814; Q2M2T1; Q64478; Q93079; P58876; Q6ZWY9; P62807; P33778; Q00729; P06900; P02281; NP_066402.2

Format

Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
13904
Recommended Dilutions
IHC: 1:50-100; FC: 1:10-50; WB: 1:1000
HIST1H2BJ antibody (Center) (Cat# 102-10774) immunohistochemistry analysis in formalin fixed and paraffin embedded human hepatocarcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the HIST1H2BJ antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
HIST1H2BJ Antibody (Center) (Cat# 102-10774) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
HIST1H2BJ Antibody (Center) (Cat# 102-10774) western blot analysis in CEM,HL-60,NCI-H460 cell line lysates (35ug/lane).This demonstrates the HIST1H2BJ antibody detected the HIST1H2BJ protein (arrow).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.