3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-HIST1H3A Antibody
Catalog #: 144-61551
Phospho-Histone HIST1H3A-T3 pAb
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P68431 |
| Gene Id | 8350 |
| Gene Symbols | HIST1H3A |
| Protein Name / Synonyms | HIST1H3A, H3/A, H3FA, histone H3.1 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Research Area | Cancer |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
15kDa
Observed MW
17kDa
Immunogen
A synthetic phosphorylated peptide around T3 of human HIST1H3A (NP_003520.1).
Modification
Phospho T3
Storage
-20°C
Images
Western blot analysis of extracts of C6 cells, using Phospho-HIST1H3A-T3 pAb (144-61551).
C6 cells were treated by Nocodazole (50ng/ml) for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot analysis of extracts of various cell lines, using Phospho-HIST1H3A-T3 pAb (144-61551).
293T cells were treated by Nocodazole (50ng/ml) for 20 hours.RAW264.7 cells were treated by Nocodazole (50ng/ml) for 20 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 1s.
Expiration:
12 months from the date of shipment when stored properly.