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Anti-HIST3H3 Antibody
Catalog #: 144-61547
Phospho-Histone H3-S28 pAb
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q16695 |
| Gene Id | 8350 |
| Gene Symbols | HIST3H3 |
| Protein Name / Synonyms | HIST1H3A, H3/A, H3FA, H3.4, H3/g, H3FT, H3t, histone H3.1 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
15kDa
Observed MW
17kDa
Immunogen
A synthetic phosphorylated peptide around S28 of human Histone H3 (NP_003520.1).
Modification
Phospho S28
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using Phospho-Histone H3-S28 antibody (144-61547) at 1:1000 dilution. Both HeLa cells and NIH/3T3 cells were treated by Calyculin A (100 nM) at 37? for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 1s.
Expiration:
12 months from the date of shipment when stored properly.