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Anti-Histamine H2 Receptor (N-term) Antibody
Catalog #: 107-10734
Histamine H2 Receptor antibody [N1], N-Terminus, 0.75 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 3274 |
| Protein Name / Synonyms | Histamine H2 Receptor, N-term |
| Concentration (lot specific) | 0.75 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
H2R antibody, HRH2 antibody, gastric receptor 1 antibody, histamine H2 receptor antibody, HH2R antibody, gastric receptor I antibody, histamine receptor H2 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
40 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 1 and 45 of Histamine H2 Receptor (Uniprot ID# P25021)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed HeLa, using Histamine H2 Receptor(107-10734) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded H520 xenograft, using Histamine H2 Receptor(107-10734) antibody at 1:100 dilution.
Histamine H2 Receptor antibody [N1], N-term detects HRH2 protein by Western blot analysis.
A. 30 µg U87-MG whole cell lysate/extract
B. 30 µg SK-N-SH whole cell lysate/extract
10 % SDS-PAGE
Histamine H2 Receptor antibody [N1], N-term (107-10734) dilution: 1:1000
A. 30 µg U87-MG whole cell lysate/extract
B. 30 µg SK-N-SH whole cell lysate/extract
10 % SDS-PAGE
Histamine H2 Receptor antibody [N1], N-term (107-10734) dilution: 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.