Anti-Histone H2A.Z Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Gene Id	3015
Protein Name / Synonyms	Histone H2A.Z
Concentration (lot specific)	0.52 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

H2A.z antibody, H2A/z antibody, H2AZ antibody, MGC117173 antibody, H2AFZ antibody, H2AZ histone antibody, histone H2A.Z antibody, "H2A histone family, member Z antibody"

Specificity

Species Reactivity

Human

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

14 kDa

Immunogen

Synthetic peptide corresponding to a region within amino acids 1 and 45 of Histone H2A.Z

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using Histone H2A.Z(107-10753) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled at 1:500.

Immunohistochemical analysis of paraffin-embedded SCC4 xenograft, using Histone H2A.Z(107-10753) antibody at 1:100 dilution.

Sample (30 ug of whole cell lysate)
A: JurKat
15% SDS PAGE
107-10753 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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