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Anti-Histone H3.3B Antibody
Catalog #: 107-11165
Histone H3.3B antibody, 0.44 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 3021 |
| Protein Name / Synonyms | Histone H3.3B |
| Concentration (lot specific) | 0.44 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
H3.3B antibody, H3F3A antibody, H3F3B antibody, histone H3.3 antibody, H3 histone, family 3A antibody, H3 histone, family 3B (H3.3B) antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
15 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 86 (P84243) of Histone H3.3B
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Histone H3.3B(107-11165) antibody at 1:200 dilution.
Immunohistochemical analysis of paraffin-embedded HSC3 xenograft, using Histone H3.3B(107-11165) antibody at 1:500 dilution.
Sample (50 ug of whole cell lysate)
A: mouse brain
15% SDS PAGE
107-11165 diluted at 1:1000
A: mouse brain
15% SDS PAGE
107-11165 diluted at 1:1000
Histone H3.3B antibody detects H3F3B protein by Western blot analysis.
A. 50 µg rat brain lysate/extract
15 % SDS-PAGE
Histone H3.3B antibody (107-11165) dilution: 1:1000
A. 50 µg rat brain lysate/extract
15 % SDS-PAGE
Histone H3.3B antibody (107-11165) dilution: 1:1000
Histone H3.3B antibody detects Histone H3.3B protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3.3B protein stained by Histone H3.3B antibody (107-11165) diluted at 1:500.
Blue: Hoechst 33342 staining.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Histone H3.3B protein stained by Histone H3.3B antibody (107-11165) diluted at 1:500.
Blue: Hoechst 33342 staining.
Histone H3.3B antibody detects Histone H3.3B protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
Histone H3.3B antibody (107-11165) dilution: 1:500.
Sample: Paraffin-embedded mouse colon.
Histone H3.3B antibody (107-11165) dilution: 1:500.
Histone H3.3B antibody detects Histone H3.3B protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
Histone H3.3B antibody (107-11165) dilution: 1:500.
Sample: Paraffin-embedded mouse prostate.
Histone H3.3B antibody (107-11165) dilution: 1:500.
Sample (30 ug of whole cell lysate)
A: Hela
15% SDS PAGE
107-11165 diluted at 1:1000
A: Hela
15% SDS PAGE
107-11165 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.