Anti-HLA-B (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P03989
Gene Symbols	HLA-B
Protein Name / Synonyms	HLA-B (N-term)
Clonality	Polyclonal
Preparation	This HLA-B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-90 amino acids from the N-terminal region of human HLA-B.
Concentration (lot specific)	0.500 mg/ml
Recommended Applications	Western Blotting, FC
Research Area	Inflammation
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

HLA-B is involved in the presentation of foreign antigens to the immune system.

Specificity

Other Names

HLA-B; HLAB; HLA class I histocompatibility antigen, B-27 alpha chain; MHC class I antigen B*27

Other Accession #

P30490; P30488; P30487; P30486; P30485; P30483; P30481; P30479; Q04826; P18463; P30464; P30461

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

40428

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; FC: 1:10-50

	Western blot analysis of lysates from CEM, HL-60, Jurkat, Ramos cell line (from left to right), using HLA-B Antibody (N-term)(Cat# 102-17948). 102-17948 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
	Western blot analysis of lysates from HL-60, Jurkat, Ramos cell line (from left to right), using HLA-B Antibody (N-term)(Cat# 102-17948). 102-17948 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
	Western blot analysis of HLA-B Antibody (N-term) (Cat# 102-17948) in A2058 cell line lysates (35ug/lane). HLA-B (arrow) was detected using the purified Pab.
	Western blot analysis of HLA-B Antibody (N-term) (Cat# 102-17948) in A375 cell line lysates (35ug/lane). HLA-B (arrow) was detected using the purified Pab.
	Western blot analysis of HLA-B (arrow) using rabbit polyclonal HLA-B Antibody (N-term) (Cat# 102-17948). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-B gene.
	Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with HLA-B Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	HLA-B Antibody (N-term) (Cat# 102-17948) flow cytometry analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.