RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

www.RayBiotech.com | info@raybiotech.com

770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-HLA B27 Antibody, FITC

Catalog #: DS-MB-03307

Mouse Anti-Human HLA B27 [+FITC]

Product Description

Specifications

Size100 tests
Estimated Lead Time1-2 weeks
SpeciesHuman
Specificity
This product is a cytotoxic antibody which detects HLA-B27 alloantigen. This antibody reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA-B27 + and precipitates cell surface molecules of 43 and 12 kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin.

Affinity studies by Scatchard analysis showed that this antibody has a higher affinity for HLA-B27 (9.7 x 108M-1) than for HLA B7 (9.5 x 107 M-1).

Subjects Expected Fluorescence Intensity Heterozygous HLA-B27+ Strong Homozygous HLA-B27+ Strong Heterozygous HLA-B7+ Faint Non B27, Non B7 Negative
Host SpeciesMouse
Protein Name / Synonyms
HLA B27
Concentration (lot specific)
50 ug/ml
Recommended ApplicationsFlow Cytometry
Shipping TypeBlue ice
Storage4°C

Description

Storage/Stability

Store at +4 °C.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Formulation:
Phosphate buffered saline

Preparation

Immunogen:
Immune complex precipitated from HLA-B27 positive cell line (Bordin) by anti HLA-antibody and Staphylococcal Protein A.
Preservative:
0.09%Sodium Azide
0.2%Bovine Serum Albumin
Expiration:
12 months from the date of shipment when stored properly.