HLA-DMA antibody, 1 mg/ml (100 ul)
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Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 3108 |
| Protein Name / Synonyms | HLA-DMA |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Synonyms
D6S222E antibody, DMA antibody, HLADM antibody, RING6 antibody, HLA-DMA antibody, MHC class II antigen DMA antibody, "class II histocompatibility antigen, M alpha chain antibody", really interesting new gene 6 protein antibody, "HLA class II histocompatibility antigen, DM alpha chain antibody", "major histocompatibility complex, class II, DM alpha antibody"
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
29
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 52 and 245 of HLA-DMA
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded Breast ca, using HLA-DMA(107-11143) antibody at 1:250 dilution.
HLA-DMA antibody detects HLA-DMA protein by Western blot analysis.
A. 30 µg Jurkat whole cell lysate/extract
B. 30 µg Raji whole cell lysate/extract
12 % SDS-PAGE
HLA-DMA antibody (107-11143) dilution: 1:500
A. 30 µg Jurkat whole cell lysate/extract
B. 30 µg Raji whole cell lysate/extract
12 % SDS-PAGE
HLA-DMA antibody (107-11143) dilution: 1:500
Sample (30 ug of whole cell lysate)
A: Hela
12% SDS PAGE
107-11143 diluted at 1:1000
A: Hela
12% SDS PAGE
107-11143 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.