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Anti-HLA-DRB1 (N-term) Antibody
Catalog #: 102-16993
Rabbit Anti-Human HLA-DRB1 (N-term) Antibody, 400 ul
Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P04229 |
| Gene Symbols | HLA-DRB1 |
| Protein Name / Synonyms | HLA-DRB1 (N-term) |
| Clonality | Polyclonal |
| Preparation | This HLA-D_1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 30-58 amino acids from the N-terminal region of human HLA-D_1. |
| Concentration (lot specific) | 0.360 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, FC |
| Research Area | Cancer, Inflammation, Autoimmune Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Introduction
HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons.
Specificity
Other Names
HLA-DRB1; HLA class II histocompatibility antigen, DRB1-1 beta chain; MHC class II antigen DRB1*1
Other Accession #
Q30167
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
29914
Recommended Dilutions
WB: 1:1000; IHC: 1:10-50; FC: 1:10-50
 | Western blot analysis of HLA-DRB1 Antibody (N-term) (Cat# 102-16993) in WiDr cell line lysates (35ug/lane). HLA-DRB1 (arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human brain tissue reacted with HLA-DRB1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | Flow cytometric analysis of widr cells using HLA-DRB1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.