Anti-HLA-G (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P17693
Gene Id	3135
Gene Symbols	HLA-G
Protein Name / Synonyms	HLA-G (Center)
Clonality	Polyclonal
Preparation	This HLA-G antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 62-89 amino acids from the Central region of human HLA-G.
Concentration (lot specific)	0.360 mg/ml
Recommended Applications	Western Blotting, IHC-P, FC
Research Area	Angiogenesis
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail.

Specificity

Other Names

HLA-G; HLA-6.0; HLAG; HLA class I histocompatibility antigen, alpha chain G; HLA G antigen; MHC class I antigen G

NCBI Accession #

NP_002118.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

38224

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; FC: 1:10-50

	Western blot analysis of lysate from human placenta tissue lysate, using HLA-G Antibody (Center))(Cat# 102-18087). 102-18087 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 35ug per lane.
	Western blot analysis of HLA-G Antibody (Center) (Cat# 102-18087) in NCI-H460 cell line lysates (35ug/lane). HLA-G (arrow) was detected using the purified Pab.
	Western blot analysis of HLA-G (arrow) using rabbit polyclonal HLA-G Antibody (Center) (Cat# 102-18087). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HLA-G gene.
	Formalin-fixed and paraffin-embedded human lung carcinoma reacted with HLA-G Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
	HLA-G Antibody (Center) (Cat# 102-18087) flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.