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Anti-HMGCL Antibody
Catalog #: 107-10814
HMGCL antibody, 1 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 3155 |
| Protein Name / Synonyms | HMGCL |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
HL antibody, HMGCL antibody, mitochondrial 3-hydroxy-3-methylglutaryl-CoA lyase antibody, HMG-CoA lyase antibody, 3-hydroxy-3-methylglutaryl-CoA lyase antibody, hydroxymethylglutaryl-CoA lyase, mitochondrial antibody, hydroxymethylglutaricaciduria antibody, 3-hydroxy-3-methylglutarate-CoA lyase antibody, 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase antibody, 3-hydroxymethyl-3-methylglutaryl-CoA lyase antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
34 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 1 and 325 of HMGCL (Uniprot ID# P35914)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:1000-1:10000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using HMGCL(107-10814) antibody at 1:500 dilution.
HMGCL antibody detects HMGCL protein by Western blot analysis.
A. 50 µg mouse liver lysate/extract
10 % SDS-PAGE
HMGCL antibody (107-10814) dilution: 1:10000
A. 50 µg mouse liver lysate/extract
10 % SDS-PAGE
HMGCL antibody (107-10814) dilution: 1:10000
HMGCL antibody detects HMGCL protein by Western blot analysis.
A. 50 µg rat liver lysate/extract
10 % SDS-PAGE
HMGCL antibody (107-10814) dilution: 1:10000
A. 50 µg rat liver lysate/extract
10 % SDS-PAGE
HMGCL antibody (107-10814) dilution: 1:10000
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using HL(107-10814) antibody at 1:200 dilution.
Sample (30 ug of whole cell lysate)
A: H1299
10% SDS PAGE
107-10814 diluted at 1:3000
A: H1299
10% SDS PAGE
107-10814 diluted at 1:3000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.