Plasmid vectors are commonly used for the expression of hybrid fusion proteins, encoding a protein of interest fused to an epitope tag. Antibodies specific for such fusion protein tags are useful for monitoring expression of the protein of interest, both in cells and in Western blot analysis. Such epitope tags in common use include Myc, GST, His, HA and GFP. HN is a novel series of epitope tags. The tags are encoded by the gateway expression vectors(from Clontech), a group of unique and effective vectors suitable for the production of recombinant tagged fusion proteins. Each expression vector encodes a unique, 12 amino acid HN-Tag epitope sequence. Anti-HN-Tag antibodies are suitable for purification, detection and immunoprecipitation of recombinant fusion proteins containing the respective HN-Tag.

The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.

HN- Tag antibody detects fusion recombinant proteins derived from a varity of sources at various concentrations. No Cross reactivity was not found in ELISA and Western Blot..