RayBiotech

3607 Parkway Ln Ste 200 Norcross, Georgia 30092

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770-729-2992 | +1-888-494-8555

ISO 13485:2016 cGMP

Anti-HN tag Antibody

Catalog #: RB-10-0003

Rabbit Anti-HN tag Antibody

Product Description

Specifications

Size20 µg, 100 µg, 200 µg, 500 µg
Estimated Lead Time1-2 business days
Specificity
HN-Tag antibody detects fusion recombinant proteins derived from a varity of sources at various concentrations. No Cross reactivity was not found in ELISA and Western Blot.
ClonalityPolyclonal
Immunogen
Conjugated-Peptide with sequence -CHNHNHNHNHNHNHN, was used as immunogen. The antibody was purified from rabbit serum by ammonium sulphate precipitation followed by antigen affinity chromatography.
Immunogen Sequence
CGPGHNHNHNHNHNHNR
Recommended ApplicationsELISA (recommended work dilution= 1:1000-5000), Western Blotting( recommended work dilution= 1-2 ug/ml)
Reconstitution
Lyophilized rabbit anti-HN-Tag antibody is stable at 4°C and below -18°C. Supplied as 100 µg of purified antibody. Reconstitute with sterile water. If 1 ml of water is used, the antibody concentration will be 100 µg/ml. Please avoid freeze-thaw cycles.
Shipping TypeAmbient temperature
Storage-20°C

Description

Description

Plasmid vectors are commonly used for the expression of hybrid fusion proteins, encoding a protein of interest fused to an epitope tag. Antibodies specific for such fusion protein tags are useful for monitoring expression of the protein of interest, both in cells and in Western blot analysis. Such epitope tags in common use include Myc, GST, His, HA and GFP. HN is a novel series of epitope tags. The tags are encoded by the gateway expression vectors(from Clontech), a group of unique and effective vectors suitable for the production of recombinant tagged fusion proteins. Each expression vector encodes a unique, 12 amino acid HN-Tag epitope sequence. Anti-HN-Tag antibodies are suitable for purification, detection and immunoprecipitation of recombinant fusion proteins containing the respective HN-Tag.

Specificity

HN- Tag antibody detects fusion recombinant proteins derived from a varity of sources at various concentrations. No Cross reactivity was not found in ELISA and Western Blot..

Storage

Lyophilized samples are stable for a year at least from date of receipt when stored at -20° C to -70° C. Upon reconstitution, the antibody can be stored at 2° - 8° C for at least a month without detectable loss of activity. Minimize freezing and thawing.
Expiration:
12 months from the date of shipment when stored properly.