3607 Parkway Ln Ste 200 Norcross, Georgia 30092
www.RayBiotech.com | info@raybiotech.com
770-729-2992 | +1-888-494-8555
ISO 13485:2016 cGMP
Anti-HNF4A Antibody
Catalog #: 144-02085
HNF4A Polyclonal Antibody
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | P41235 |
| Gene Id | 3172 |
| Gene Symbols | HNF4A |
| Protein Name / Synonyms | HNF4A, FRTS4, HNF4, HNF4a7, HNF4a8, HNF4a9, HNF4alpha, MODY, MODY1, NR2A1, NR2A21, TCF, TCF14, hepatocyte nuclear factor 4-alpha |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Background
The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IHC 1:50 - 1:200
Format
Calculated MW
43-56kDa
Observed MW
53kDa
Immunogen
A synthetic peptide of human HNF4A
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using HNF4A antibody (144-02085) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat pancreas using HNF4A Antibody (144-02085) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using HNF4A Antibody (144-02085) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using HNF4A Antibody (144-02085) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat pancreas using HNF4A Antibody (144-02085) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using HNF4A Antibody (144-02085) at dilution of 1:100 (40x lens).
Expiration:
12 months from the date of shipment when stored properly.