Anti-HNF4A (Center) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Rat
Host Species	Rabbit
Accession Number	P41235
Gene Id	3172
Gene Symbols	HNF4A
Protein Name / Synonyms	HNF4A (Center)
Clonality	Polyclonal
Preparation	This HNF4A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 281-312 amino acids from the Central region of human HNF4A.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IHC-P, IF, FC
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

HNF4A is a nuclear transcription factor which binds DNA as a homodimer. This protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This protein may play a role in development of the liver, kidney, and intestines. Mutations in HNF4A gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I.

Specificity

Predicted Reactivity

Mouse

Other Names

HNF4A; HNF4; NR2A1; TCF14; Hepatocyte nuclear factor 4-alpha; Nuclear receptor subfamily 2 group A member 1; Transcription factor 14; Transcription factor HNF-4

NCBI Accession #

NP_000448.3;NP_001025174.1;NP_001025175.1;NP_001245284.1;NP_787110.2;NP_849181.1

Other Accession #

P22449; P49698

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

52785

Recommended Dilutions

WB: 1:1000; IHC: 1:10-50; IF: 1:10-50; FC: 1:10-50

	HNF4A Antibody (Center) (Cat# 102-15136) western blot analysis in 293,HepG2 cell line and rat liver tissue lysates (35ug/lane).This demonstrates the HNF4A antibody detected the HNF4A protein (arrow).
	HNF4A Antibody (Center) (102-15136)immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HNF4A Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
	Confocal immunofluorescent analysis of HNF4A Antibody (Center)(Cat# 102-15136) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
	HNF4A Antibody (Center) (Cat# 102-15136) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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