Anti-HNMT (N-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P50135
Gene Id	3176
Gene Symbols	HNMT
Protein Name / Synonyms	HNMT (N-term)
Clonality	Polyclonal
Preparation	This HNMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-58 amino acids from the N-terminal region of human HNMT.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	IF, Western Blotting, IHC-P
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

In mammals, histamine is metabolized by two major pathways: N(tau)-methylation via histamine N-methyltransferase and oxidative deamination via diamine oxidase. HNMT is the first enzyme which is found in the cytosol and uses S-adenosyl-L-methionine as the methyl donor. In the mammalian brain, the neurotransmitter activity of histamine is controlled by N(tau)-methylation as diamine oxidase is not found in the central nervous system. A common genetic polymorphism affects the activity levels of this gene product in red blood cells.

Specificity

Other Names

HNMT; Histamine N-methyltransferase

NCBI Accession #

NP_001019245.1;NP_001019246.1;NP_008826.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

33295

Recommended Dilutions

IF: 1:10-50; WB: 1:1000; IHC: 1:10-50

	Confocal immunofluorescent analysis of HNMT Antibody (N-term)(Cat# 102-12484) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).
	Western blot analysis of HNMT Antibody (N-term) (Cat# 102-12484) in Hela cell line lysates (35ug/lane). HNMT(arrow) was detected using the purified polyclonal antibody.
	Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with HNMT antibody (N-term) (Cat# 102-12484), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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