Anti-hnRNP C1/C2 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse, Rat
Host Species	Rabbit
Gene Id	3183
Protein Name / Synonyms	hnRNP C1/C2
Concentration (lot specific)	0.79 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

C1 antibody, C2 antibody, HNRNP antibody, HNRPC antibody, MGC104306 antibody, MGC105117 antibody, MGC117353 antibody, MGC131677 antibody, SNRPC antibody, HNRNPC antibody, hnRNP C1/C2 antibody, nuclear ribonucleoprotein particle C1 protein antibody, heterogeneous nuclear ribonucleoproteins C1/C2 antibody, nuclear ribonucleoprotein particle C2 protein antibody, heterogeneous nuclear ribonucleoprotein C (C1/C2) antibody

Specificity

Species Reactivity

Human, Rat, Mouse

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

34 kDa

Immunogen

Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 142 (P07910) of hnRNP C1/C2

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

hnRNP C1/C2 antibody detects HNRNPC protein by Western blot analysis.
A. 30 µg Neuro2A whole cell lysate/extract
B. 30 µg NIH-3T3 whole cell lysate/extract
C. 30 µg BCL-1 whole cell lysate/extract
D. 30 µg Raw264.7 whole cell lysate/extract
E. 30 µg C2C12 whole cell lysate/extract
12 % SDS-PAGE
hnRNP C1/C2 antibody (107-11067) dilution: 1:1000

hnRNP C1/C2 antibody detects HNRNPC protein by Western blot analysis.
A. 30 µg PC-12 whole cell lysate/extract
B. 30 µg Rat2 whole cell lysate/extract
12 % SDS-PAGE
hnRNP C1/C2 antibody (107-11067) dilution: 1:1000

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using hnRNP C1/C2 (107-11067) antibody at 1:200 dilution.

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft, using hnRNP C1/C2 (107-11067) antibody at 1:100 dilution.

Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
107-11067 diluted at 1:1000

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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