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Anti-Homer Antibody
Catalog #: 107-10378
Homer antibody, 1 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Gene Id | 9456 |
| Protein Name / Synonyms | Homer |
| Concentration (lot specific) | 1 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
HOMER antibody, HOMER1A antibody, HOMER1B antibody, HOMER1C antibody, SYN47 antibody, Ves-1 antibody, HOMER1 antibody, homer protein homolog 1 antibody, homer, neuronal immediate early gene, 1 antibody, homer-1 antibody, homer homolog 1 (Drosophila) antibody
Specificity
Species Reactivity
Human, Rat, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
40 kDa
Immunogen
Recombinant fragment corresponding to a region within amino acids 72 and 339 of Homer (Uniprot ID# Q86YM7)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Immunofluorescence analysis of methanol-fixed HeLa, using Homer(107-10378) antibody at 1:500 dilution.
Homer antibody detects HOMER1 protein by Western blot analysis.
A. 30 µg IMR32 whole cell lysate/extract
10 % SDS-PAGE
Homer antibody (107-10378) dilution: 1:1000
A. 30 µg IMR32 whole cell lysate/extract
10 % SDS-PAGE
Homer antibody (107-10378) dilution: 1:1000
Sample (20 ug of whole cell lysate)
A: mouse brain
10% SDS PAGE
107-10378 diluted at 1:20000
A: mouse brain
10% SDS PAGE
107-10378 diluted at 1:20000
Homer antibody detects HOMER1 protein by Western blot analysis.
A. 50 µg rat brain lysate/extract
10 % SDS-PAGE
Homer antibody (107-10378) dilution: 1:500
A. 50 µg rat brain lysate/extract
10 % SDS-PAGE
Homer antibody (107-10378) dilution: 1:500
Homer antibody detects Homer protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10 % SDS-PAGE, and blotted with Homer antibody (107-10378) diluted by 1:1000
Immunohistochemical analysis of paraffin-embedded OVCA, using HOMER1(107-10378) antibody at 1:100 dilution.
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.