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Anti-HOMEZ (N-term) Antibody

Catalog #: 102-10796

Rabbit Anti-HOMEZ (N-term) Antibody, 400 ul

Product Description

Specifications

Size400 µL
Estimated Lead Time1-2 weeks
SpeciesHuman, Mouse
Host SpeciesRabbit
Accession Number
Q8IX15
Gene Id
57594
Gene Symbols
HOMEZ
Protein Name / Synonyms
HOMEZ (N-term)
ClonalityPolyclonal
PreparationThis HOMEZ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 158-186 amino acids from the N-terminal region of human HOMEZ.
Concentration (lot specific)
0.5 mg/ml
Recommended ApplicationsWestern Blotting, FC, IF
Shipping TypeBlue ice
Storage-20°C

Description

Introduction

May function as a transcriptional regulator.

Specificity

Other Names
HOMEZ; KIAA1443; Homeobox and leucine zipper protein Homez; Homeodomain leucine zipper-containing factor
NCBI Accession #
NP_065885.2
Other Accession #
NP_065885.2

Format

Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
61240
Recommended Dilutions
WB: 1:1000; FC: 1:10-50; IF: 1:10-50
HOMEZ Antibody (N-term) (Cat# 102-10796) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the HOMEZ antibody detected the HOMEZ protein (arrow).
HOMEZ Antibody (N-term) (Cat# 102-10796) flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Fluorescent confocal image of A431 cell stained with HOMEZ Antibody (N-term)(Cat# 102-10796).A431 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOMEZ primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HOMEZ immunoreactivity is localized to Nucleus significantly.

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.