Anti-HOXD8 (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human
Host Species	Rabbit
Accession Number	P13378
Gene Id	3234
Gene Symbols	HOXD8
Protein Name / Synonyms	HOXD8 (C-term)
Clonality	Polyclonal
Preparation	This HOXD8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-270 amino acids from the C-terminal region of human HOXD8.
Concentration (lot specific)	0.5 mg/ml
Recommended Applications	IF, Western Blotting
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene belongs to the homeobox family of genes. The homeobox genes encode a highly conserved family of transcription factors that play an important role in morphogenesis in all multicellular organisms. Mammals possess four similar homeobox gene clusters, HOXA, HOXB, HOXC and HOXD, located on different chromosomes, consisting of 9 to 11 genes arranged in tandem. This gene is one of several homeobox HOXD genes located in a cluster on chromosome 2. Deletions that remove the entire HOXD gene cluster or the 5' end of this cluster have been associated with severe limb and genital abnormalities. In addition to effects during embryogenesis, this particular gene may also play a role in adult urogenital tract function.

Specificity

Other Names

HOXD8; HOX4E; Homeobox protein Hox-D8; Homeobox protein Hox-4E; Homeobox protein Hox-5.4

NCBI Accession #

NP_001186675.1;NP_062458.1

Other Accession #

NP_062458.1

Format

Type

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

31911

Recommended Dilutions

IF: 1:10-50; WB: 1:1000

Fluorescent image of HUVEC cell stained with HOXD8 Antibody (C-term)(Cat# 102-13985).HUVEC cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HOXD8 primary antibody (1:25, 1 h at 37^°). For secondary antibody, Alexa Fluor^® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37^°).Cytoplasmic actin was counterstained with Alexa Fluor^® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37^°).HOXD8 immunoreactivity is localized to Nucleus significantly.

HOXD8 Antibody (C-term)(Cat# 102-13985) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the HOXD8 antibody detected the HOXD8 protein (arrow).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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