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Anti-HPS3 (C-term) Antibody
Catalog #: 107-10710
HPS3 antibody [C3], C-Terminus, 0.92 mg/ml (100 ul)
Product Description
Specifications
| Size | 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Gene Id | 84343 |
| Protein Name / Synonyms | HPS3, C-term |
| Concentration (lot specific) | 0.92 mg/ml |
| Recommended Applications | ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot |
| Shipping Type | Blue ice |
| Storage | -20°C |
Description
Synonyms
DKFZp686F0413 antibody, FLJ22704 antibody, SUTAL antibody, HPS3 antibody, Hermansky-Pudlak syndrome 3 protein antibody, Hermansky-Pudlak syndrome 3 antibody
Specificity
Species Reactivity
Human
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
114 kDa
Immunogen
Synthetic peptide corresponding to a region within amino acids 942 and 1004 of HPS3 (Uniprot ID# Q969F9)
Applications
Applications Tested
ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
HPS3 antibody [C3], C-term detects HPS3 protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HPS3 protein stained by HPS3 antibody [C3], C-term (107-10710) diluted at 1:500.
Blue: Hoechst 33343 staining.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: HPS3 protein stained by HPS3 antibody [C3], C-term (107-10710) diluted at 1:500.
Blue: Hoechst 33343 staining.
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using HPS3(107-10710) antibody at 1:500 dilution.
Sample(30 µg of whole cell lysate)
A:293T
B:A431
C:H1299
D:HeLa S3
E:Hep G2
F:MOLT4
G:Raji
7.5% SDS PAGE
107-10710 diluted at 1:1000
A:293T
B:A431
C:H1299
D:HeLa S3
E:Hep G2
F:MOLT4
G:Raji
7.5% SDS PAGE
107-10710 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.