Anti-HRAS (C-term) Antibody

Product Description

Specifications

Size	400 µL
Estimated Lead Time	1-2 weeks
Species	Human, Mouse
Host Species	Rabbit
Accession Number	P01112
Gene Id	3265
Gene Symbols	HRAS
Protein Name / Synonyms	HRAS (C-term)
Clonality	Polyclonal
Preparation	This HRAS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 146-176 amino acids from the C-terminal region of human HRAS.
Concentration (lot specific)	2.0 mg/ml
Recommended Applications	Western Blotting, IF
Research Area	Cardiovascular Disease, Cancer
Shipping Type	Blue ice
Storage	-20°C

Description

Introduction

This gene belongs to the Ras oncogene family, whose members are related to the transforming genes of mammalian sarcoma retroviruses. The products encoded by these genes function in signal transduction pathways. These proteins can bind GTP and GDP, and they have intrinsic GTPase activity. This protein undergoes a continuous cycle of de- and re-palmitoylation, which regulates its rapid exchange between the plasma membrane and the Golgi apparatus. Mutations in this gene cause Costello syndrome, a disease characterized by increased growth at the prenatal stage, growth deficiency at the postnatal stage, predisposition to tumor formation, mental retardation, skin and musculoskeletal abnormalities, distinctive facial appearance and cardiovascular abnormalities. Defects in this gene are implicated in a variety of cancers, including bladder cancer, follicular thyroid cancer, and oral squamous cell carcinoma. Multiple transcript variants, which encode different isoforms, have been identified for this gene.

Specificity

Predicted Reactivity

Rat, Chicken

Other Names

HRAS; HRAS1; GTPase HRas; H-Ras-1; Ha-Ras; Transforming protein p21; c-H-ras; p21ras; GTPase HRas, N-terminally processed

NCBI Accession #

NP_001123914.1;NP_005334.1;NP_789765.1

Other Accession #

P20171; Q61411; P08642; NP_005334.1

Format

Type

Purified Rabbit Polyclonal Antibody (Pab)

Calculated Molecular Weight (Da)

21298

Recommended Dilutions

WB: 1:1000; IF: 1:10-50

	Western blot analysis of HRAS (arrow) using rabbit polyclonal HRAS Antibody (C-term) (Cat# 102-11199). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the HRAS gene.
	HRAS Antibody (C-term) (Cat# 102-11199) western blot analysis in mouse cerebellum tissue lysates (35ug/lane).This demonstrates the HRAS antibody detected the HRAS protein (arrow).
	Confocal immunofluorescent analysis of HRAS Antibody (C-term)(Cat# 102-11199) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)

Expiration:

12 months from the date of shipment when stored properly.

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