Anti-HSD17B4 Antibody

Product Description

Specifications

Size	100 µL
Estimated Lead Time	1-2 weeks
Species	Human, Rat
Host Species	Rabbit
Gene Id	3295
Protein Name / Synonyms	HSD17B4
Concentration (lot specific)	1 mg/ml
Recommended Applications	ICC/IF, IHC (Formalin-fixed paraffin-embedded sections), Western blot
Shipping Type	Blue ice
Storage	-20°C

Description

Synonyms

DBP antibody, MFE-2 antibody, MPF-2 antibody, SDR8C1 antibody, HSD17B4 antibody, "short chain dehydrogenase/reductase family 8C, member 1 antibody", peroxisomal multifunctional protein 2 antibody, 17beta-estradiol dehydrogenase type IV antibody, D-3-hydroxyacyl-CoA dehydratase antibody, 17-beta-HSD IV antibody, beta-keto-reductase antibody, multifunctional protein 2 antibody, 17-beta-HSD 4 antibody, peroxisomal multifunctional enzyme type 2 antibody, beta-hydroxyacyl dehydrogenase antibody, "3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase antibody", 17-beta-hydroxysteroid dehydrogenase 4 antibody, "D-bifunctional protein, peroxisomal antibody", hydroxysteroid (17-beta) dehydrogenase 4 antibody

Specificity

Species Reactivity

Human, Rat

Format

Product Type

Primary antibody

Formulation

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Calculated Molecular Weight

80 kDa

Immunogen

Recombinant fragment corresponding to a region within amino acids 355 and 645 of HSD17B4 (Uniprot ID# P51659)

Applications

Applications Tested

ICC/IF,IHC (Formalin-fixed paraffin-embedded sections),Western blot

Application Notes

Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000[suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunofluorescence analysis of methanol-fixed Hep3B, using HSD17B4(107-10409) antibody at 1:500 dilution.

HSD17B4 antibody detects HSD17B4 protein by Western blot analysis.
A. 20 ug Rat heart lysate/extract
7.5% SDS-PAGE
HSD17B4 antibody (107-10409) dilution: 1:3000

Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft , using HSD17B4(107-10409) antibody at 1:500 dilution.

Storage

Keep as concentrated solution. Aliquot and store at -20^°C or below. Avoid multiple freeze-thaw cycles.

Expiration:

12 months from the date of shipment when stored properly.

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